Application
Western blot analysis of GPAA1 expression in non-transfected 293T: (A), human GPAA1 transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | GPAA1 Antibody (H-311) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 46-356 mapping near the N-terminus of GPAA1 of human origin |
| Modification Notes | Glycosylphosphatidylinositol (GPI) acts as a membrane anchor for cell surface proteins. Glycosylphosphatidylinositol anchor attachment 1 protein (GPAA1), also designated GPI anchor attachment protein 1 or GAA1 protein homolog, is a membrane protein localized to the endoplasmic reticulum which is involved in GPI-anchor biosynthesis. GPAA1 is crucial for GPI-anchoring of precursor proteins and catalyzes the attachment of GPI to proteins containing a C-terminal GPR attachment signal. GAA1 contains an N-terminal signal sequence, one cAMP- and cGMP-dependent protein kinase phosphorylation site, two potential N-glycosylation sites, one leucine zipper pattern and eight putative transmembrane domains. GPAA1 is ubiquitously expressed and shows higher levels of expression in fetal tissues than in adult tissues. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GPAA1 isoforms 1, 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 46-356 mapping near the N-terminus of GPAA1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 46-356 mapping near the N-terminus of GPAA1 of human origin |
| Entrez Gene | 8733 |
|---|
Application
Western blot analysis of GPAA1 expression in non-transfected 293T: (A), human GPAA1 transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of GPAA1 isoforms 1, 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
