Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing membrane and nuclear staining of glandular cells.Application
Western blot analysis of Glyoxalase I expression in non-transfected: (A) and human Glyoxalase I transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Glyoxalase I expression in non-transfected 293T: (A), human Glyoxalase I transfected 293T: (B) and HEL 92.1.7 (C) whole cell lysates.Application
Western blot analysis of Glyoxalase I expression in HEL 92.1.7 (A), TF-1 (B) and HCT 116 (C) whole cell lysates.| Product Name | Glyoxalase I Antibody (FL-184) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-184 representing full length Glyoxalase I of human origin |
| Modification Notes | The glyoxal pathway plays a role in the detoxification of glucose degradation products (GDP). Glyoxalase I (GLO1), a member of the glyoxalase family, is effective in eliminating GDP. Overexpression or silencing of Glyoxalase I in mice brain suggests an association between Glyoxalase I and anxiety. Glyoxalase I has three isoforms generated from two alleles in the genome which forms two homodimers and one heterodimer, each subunit binding one zinc ion. Research demonstrates that GLO1 gene expression is induced in colon carcinoma. Both an insulin response element (IRE), and a zinc metal response element (MRE) in the promoter region of the GLO1 gene have been identified. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Glyoxalase I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-184 representing full length Glyoxalase I of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-184 representing full length Glyoxalase I of human origin |
| Entrez Gene | 2739 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing membrane and nuclear staining of glandular cells.
Application
Western blot analysis of Glyoxalase I expression in non-transfected: (A) and human Glyoxalase I transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Glyoxalase I expression in non-transfected 293T: (A), human Glyoxalase I transfected 293T: (B) and HEL 92.1.7 (C) whole cell lysates.
Application
Western blot analysis of Glyoxalase I expression in HEL 92.1.7 (A), TF-1 (B) and HCT 116 (C) whole cell lysates.| Application Notes | recommended for detection of Glyoxalase I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
