Application
Western blot analysis of MAT IIa expression in non-transfected 293T: (A), mouse MAT IIa transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of MAT I, MAT II and MAT III expression in HeLa nuclear extract (A) and Hep G2-treated rat liver tissue extract (B).| Product Name | MAT I/II/III Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of MAT II of human origin |
| Modification Notes | Methionine adenosyltransferase (MAT) catalyzes the formation of S-adenosyltransferase (AdoMet) for methionine catabolism in the liver. Two different genes, MAT1A and MAT2A, encode a liver specific and non-liver specific form of MAT, respectively. Inactivation of the liver specific gene product, designated MAT I/III, associates with liver diseases such as cirrhosis. MAT1A expression also correlates with a differentiated phenotype, whereas liver cells expressing MAT2A present a dedifferentiated phenotype and lowered AdoMet synthesis. Likewise, NF-kB and TNFå cause a switch from MAT1A to MAT2A expression in human hepatocellular carcinoma (HCC), which facilitates cancer cell growth. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MAT I, II and III of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of MAT II of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of MAT II of human origin |
| Entrez Gene | 4144 |
|---|
Application
Western blot analysis of MAT IIa expression in non-transfected 293T: (A), mouse MAT IIa transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of MAT I, MAT II and MAT III expression in HeLa nuclear extract (A) and Hep G2-treated rat liver tissue extract (B).| Application Notes | recommended for detection of MAT I, II and III of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
