Application
Western blot analysis of MRTF-A expression in HeLa (A) and A549 (B) nuclear extracts.| Product Name | MRTF-A Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 761-900 mapping near the C-terminus of MRTF-A of human origin |
| Modification Notes | Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF regulates the transient response of several muscle genes in response to growth factors and recruits accessory myogenic factors to activate these muscle genes. SRF is required for the formation of vertebrate mesoderm leading to the origin of the cardiovascular system. Myocardin, in association with SRF in cardiac muscle cells, activates cardiac muscle promoters. Myocardin-related transcription factor A (MRTF-A) interacts with SRF and stimulates its transcriptional activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Myocardin-related transcription factor A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 761-900 mapping near the C-terminus of MRTF-A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 761-900 mapping near the C-terminus of MRTF-A of human origin |
| Entrez Gene | 57591 |
|---|
Application
Western blot analysis of MRTF-A expression in HeLa (A) and A549 (B) nuclear extracts.| Application Notes | recommended for detection of Myocardin-related transcription factor A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
