Application
Western blot analysis of VPAC1 expression in non-transfected: (A) and human VPAC1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of VPAC1 expression in TE671 (A), SK-N-SH (B) and Caki-1 (C) whole cell lysates.| Product Name | VPAC1 Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 31-160 mapping near the N-terminus of VPAC1 of human origin |
| Modification Notes | The vasoactive intestinal peptide (VIP) and pituitary adenylate cylase-activating polypeptide (PACAP) belong to a superfamily of peptide hormones that include glucagon, secretin and growth hormone releasing hormone (1,2). The effects of VIP and PACAP are mediated by three G-protein coupled receptors, VPAC1, VPAC2 and the PACAP receptor (also designated PAC1-R) (1,2). The VPAC receptors have equal affinities for VIP and PACAP, which stimulate the activation of adenylyl cyclase (1,3). Both VPAC1 and VPAC2 are abundantly expressed in brain and T cells, where they modulate neuronal differentiation and T cell activation, respectively (1,4-6). The PACAP receptor is a seven transmembrane protein that produces at least eight isoforms by alternative splicing (2). Each isoform is associated with a specific signaling pathway and a specific expression pattern (2). The PACAP receptor, which is thought to play an integral role in brain development, preferentially binds PACAP in order to stimulate a cAMP-protein kinase A signaling pathway (2,7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of VPAC1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 31-160 mapping near the N-terminus of VPAC1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 31-160 mapping near the N-terminus of VPAC1 of human origin |
| Entrez Gene | 7433 |
|---|
Application
Western blot analysis of VPAC1 expression in non-transfected: (A) and human VPAC1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of VPAC1 expression in TE671 (A), SK-N-SH (B) and Caki-1 (C) whole cell lysates.| Application Notes | recommended for detection of VPAC1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
