Application
Western blot analysis of SUZ12 expression in non-transfected 293: (A), human SUZ12 transfected 293: (B), HeLa (C), SW480 (D) and JEG-3 (E) whole cell lysates.| Product Name | SUZ12 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 440-739 mapping at the C-terminus of SUZ12 of human origin |
| Modification Notes | The Polycomb group (PcG) genes contribute to the maintenance of cell identity, cell cycle regulation and oncogenesis. The mammalian PcG proteins are regulatory proteins important for Hox gene expression, axial skeleton development and the control of proliferation and survival of hematopoietic cells. By inducing changes in chromatin structure, the PcG proteins are part of a cellular memory system that is responsible for gene activity being inherited to progeny cells. PcG proteins silence gene expression through the formation of multimeric protein complexes with different compositions. Manipulating the expression-levels of various PcG proteins in mammalian cell lines results in cellular transformation, which may be a link between the chromatin-associated PcG proteins and cancer. Polycomb protein SUZ12, also designated ChET 9 protein or joined-to-JAZF1 protein, is a nuclear protein belonging to the VEFS (VRN2-EMF2-FIS2-SUZ12) family. SUZ12 has been detected at the breakpoints of a certain recurrent chromosomal translocation which has been reported in endometrial stromal sarcoma. It is a component of the PRC2 complex, composed of EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SUZ12 of mouse and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 440-739 mapping at the C-terminus of SUZ12 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 440-739 mapping at the C-terminus of SUZ12 of human origin |
| Entrez Gene | 23512 |
|---|
Application
Western blot analysis of SUZ12 expression in non-transfected 293: (A), human SUZ12 transfected 293: (B), HeLa (C), SW480 (D) and JEG-3 (E) whole cell lysates.| Application Notes | recommended for detection of SUZ12 of mouse and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
