Application
Western blot analysis of SQSTM1 expression in HeLa whole cell lysate.Application
Western blot analysis of SQSTM1 expression in SK-LMS-1 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | SQSTM1 Antibody (H-290) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 151-440 of SQSTM1 of human origin |
| Modification Notes | The chronic focal skeletal disorder, Paget’s disease of bone, affects 2-3% of the population over the age of 60 years. Paget’s disease is characterized by increased bone resorption by osteoclasts, followed by abundant new bone formation that is of poor quality. The disease leads to several complications including bone pain and deformities, as well as fissures and fractures. Mutations in the ubiquitin-associated (UBA) domain of the Sequestosome 1 protein (SQSTM1), also designated p62 or ZIP, commonly cause Paget’s disease since the UBA is necessary for aggregate sequestration and cell survival. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SQSTM1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 151-440 of SQSTM1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 151-440 of SQSTM1 of human origin |
| Entrez Gene | 8878 |
|---|
Application
Western blot analysis of SQSTM1 expression in HeLa whole cell lysate.
Application
Western blot analysis of SQSTM1 expression in SK-LMS-1 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of SQSTM1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
