Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of pS2 expression in non-transfected: (A) and human pS2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of pS2 expression in MCF7 whole cell lysate.| Product Name | pS2 Antibody (FL-84) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-84 representing full length pS2 of human origin |
| Modification Notes | Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain. pS2, also designated Md2, BCEI, TFF1 and pNR-2, is a trefoil protein that is highly expressed in normal gastric mucosa. pS2 has also been detected in a number of carcinomas including cancers of the breast, pancreas and stomach. pS2 is not present in normal breast tissue but is inducible by estrogen in MCF7 cells. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. The gene encoding pS2 maps to human chromosome 21q22.3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of pS2 and, to a lesser extent, trefoil factor 2 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-84 representing full length pS2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-84 representing full length pS2 of human origin |
| Entrez Gene | 7031 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of pS2 expression in non-transfected: (A) and human pS2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of pS2 expression in MCF7 whole cell lysate.| Application Notes | recommended for detection of pS2 and, to a lesser extent, trefoil factor 2 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
