Application
Western blot analysis of Int-6 expression in 293T (A) and KNRK (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.Application
Western blot analysis of Int-6 expression in Jurkat (A), LADMAC (B) and NIH/3T3 (C) whole cell lysates.| Product Name | Int-6 Antibody (H-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 246-445 mapping at the C-terminus of Int-6 of human origin |
| Modification Notes | Int-6, also designated eIF3e, eIF3-p46, eIF3-p48 and eukaryotic translation initiation factor 3, subunit 6, regulates translation and protein degradation through binding with three complexes: the eukaryotic translation initiation factor 3 (eIF3), the proteasome regulatory lid and the constitutive photomorphogenesis 9 signalosome. eIF3 is a complex that mediates assembly of 40S ribosomal subunits on mRNA bearing either a 5'-cap or an internal ribosome entry site (IRES). The Int-6 gene is a site of mouse mammary tumour virus (MMTV) integration in murine tumors. Reducing Int-6 expression by RNA interference in HeLa cells alters mitosis progression through defects in spindle formation, chromosome segregation and cytokinesis. These abberations appear to correlate with an inhibition of cyclin B-Cdk1 kinase activity, due to a protracted inhibitory phosphorylated state of Cdk1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Int-6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 246-445 mapping at the C-terminus of Int-6 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 246-445 mapping at the C-terminus of Int-6 of human origin |
| Entrez Gene | 3646 |
|---|
Application
Western blot analysis of Int-6 expression in 293T (A) and KNRK (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
Application
Western blot analysis of Int-6 expression in Jurkat (A), LADMAC (B) and NIH/3T3 (C) whole cell lysates.| Application Notes | recommended for detection of Int-6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
