Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nucleolar, nuclear and cytoplasmic staining of squamous epithelial cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar localization.Application
Western blot analysis of Nucleostemin expression in 293T whole cell lysate.| Product Name | Nucleostemin Antibody (H-270) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 280-549 mapping at the C-terminus of Nucleostemin of human origin |
| Modification Notes | Nucleostemin, also designated Nucleolar GTP-binding protein 3, is a member of the MMR1/HSR1 GTP-binding protein family. It is expressed in the nucleoli of adult CNS stem cells, primitive bone marrow cells, embryonic stem cells and in several cancer cell lines. Nucleostemin is often used as a stem cell marker. Overexpression or depletion of the protein can reduce cell proliferation in CNS stem cells. Nucleostemin shuttles between the nucleus and the nucleolus and may be important in maintaining the proliferative capacity of stem cells. Nucleostemin is important in the growth regulation of liver cancer, gastric cancer and several other cancer types. The gene encoding Nucleostemin is localized to chromosome 3p21.1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Nucleostemin of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 280-549 mapping at the C-terminus of Nucleostemin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 280-549 mapping at the C-terminus of Nucleostemin of human origin |
| Entrez Gene | 26354 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nucleolar, nuclear and cytoplasmic staining of squamous epithelial cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar localization.
Application
Western blot analysis of Nucleostemin expression in 293T whole cell lysate.| Application Notes | recommended for detection of Nucleostemin of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
