Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar and nuclear localization.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar and cytoplasmic localization.Application
Western blot analysis of Bystin expression in non-transfected: (A) and human Bystin transfected: (B) 293T whole cell lysates.| Product Name | Bystin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 138-437 mapping at the C-terminus of Bystin of human origin |
| Modification Notes | Bystin (or Bystin-like) is an evolutionarily conserved protein from yeast to humans. It localizes to the nucleolus and cytoplasm. Nuclear Bystin associates with the 40S ribosomal subunit and cytoplasmic Bystin directly binds Trophinin and Tastin, facilitating the adhesion of cells expressing these proteins. Knockdown of Bystin inhibits cell proliferation and delays RNA processing of the 18S component of the 40S ribosomal subunit. This suggests that Bystin plays an important role in ribosome biogenesis. In addition, embryo development is arrested around the 16-cell stage when Bystin expression is knocked down. This implies that Bystin is important for the development of preimplantation embryos. Bystin is also overexpressed in human cancers providing further evidence suggesting that it participates in cell proliferation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Bystin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 138-437 mapping at the C-terminus of Bystin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 138-437 mapping at the C-terminus of Bystin of human origin |
| Entrez Gene | 705 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar and nuclear localization.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar and cytoplasmic localization.
Application
Western blot analysis of Bystin expression in non-transfected: (A) and human Bystin transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Bystin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
