Application
Western blot analysis of E-cadherin expression in ZR-75-1 (A) and LNCaP (B) whole cell lysates.Application
Western blot analysis of E-cadherin expression in F9 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human ovarian tumor (A) and immunofluorescence staining of methanol-fixed ZR-75-1 cells (B) showing membrane staining.Application
Western blot analysis of E-cadherin expression in ZR-75-1 whole cell lysate.| Product Name | E-cadherin Antibody (H-108) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against amino acids 600-707 mapping within the extracellular domain of E-cadherin of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as ∫-catenin, to regulate cadherin function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:500 IHC:1:10~1:50 IF:1:20~1:100 IP:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 600-707 mapping within the extracellular domain of E-cadherin of human origin |
|---|---|
| Tissue Specificity | raised against amino acids 600-707 mapping within the extracellular domain of E-cadherin of human origin |
| Entrez Gene | 999 |
|---|
Application
Western blot analysis of E-cadherin expression in ZR-75-1 (A) and LNCaP (B) whole cell lysates.
Application
Western blot analysis of E-cadherin expression in F9 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human ovarian tumor (A) and immunofluorescence staining of methanol-fixed ZR-75-1 cells (B) showing membrane staining.
Application
Western blot analysis of E-cadherin expression in ZR-75-1 whole cell lysate.| Application Notes | WB:1:100~1:500 IHC:1:10~1:50 IF:1:20~1:100 IP:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
