Application
Western blot analysis of Laminin-R expression in non-transfected: (A) and human Laminin-R transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Laminin-R expression in non-transfected 293T: (A), mouse Laminin-R transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of Laminin-R expression in non-transfected 293T: (A), mouse Laminin-R transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Laminin-R expression in non-transfected 293T: (A), human Laminin-R transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Laminin-R expression in non-transfected 293T: (A), human Laminin-R transfected 293T: (B) and SW480 (C) whole cell lysates.Application
Western blot analysis of Laminin-R expression in non-transfected 293T: (A), human Laminin-R transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of Laminin-R expression in non-transfected 293T: (A), human Laminin-R transfected 293T: (B) and SW480 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Laminin-R expression in HeLa (A) and Hep G2 (B) whole cell lysates.| Product Name | Laminin-R Antibody (H-141) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 110-250 mapping to an internal region of Laminin-R of human origin |
| Modification Notes | Laminin receptor (Laminin-R) has a heterodimeric structure similar to that of receptors for other extracellular matrix proteins such as Fibronectin and Vitronectin. Incorporation of Laminin-R into lysosomal membranes makes it possible for lysosomes to attach to surfaces coated with Laminin. This and other properties identify Laminin-R as a member of the integrin family of cell adhesion receptors. The Laminin-R precursor is a polypeptide whose expression is consistently upregulated in aggressive carcinoma. The precursor, which is also identified as p40 ribosome-associated protein, appears to be a multifunctional protein involved in the translational machinery. Laminin-R (also known as colon carcinoma laminin-binding protein) and is found at nine-fold higher levels in colon carcinoma than in adjacent normal colonic epithelium. Additionally, the level of the Laminin-R is higher in the lung cancer cell line than in the lung cell line. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Laminin-R of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 110-250 mapping to an internal region of Laminin-R of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 110-250 mapping to an internal region of Laminin-R of human origin |
| Entrez Gene | 3921 |
|---|
Application
Western blot analysis of Laminin-R expression in non-transfected: (A) and human Laminin-R transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Laminin-R expression in non-transfected 293T: (A), mouse Laminin-R transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of Laminin-R expression in non-transfected 293T: (A), mouse Laminin-R transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Laminin-R expression in non-transfected 293T: (A), human Laminin-R transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Laminin-R expression in non-transfected 293T: (A), human Laminin-R transfected 293T: (B) and SW480 (C) whole cell lysates.
Application
Western blot analysis of Laminin-R expression in non-transfected 293T: (A), human Laminin-R transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of Laminin-R expression in non-transfected 293T: (A), human Laminin-R transfected 293T: (B) and SW480 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Laminin-R expression in HeLa (A) and Hep G2 (B) whole cell lysates.| Application Notes | recommended for detection of Laminin-R of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
