Application
Western blot analysis of CTLA-4 expression in Jurkat (A), ALL-S1L (B), U-698-M (C), U266 (D) and CCRF-CEM (E) whole cell lysates.Application
Western blot analysis of human recombinant CTLA-4 fusion protein.| Product Name | CTLA-4 Antibody (H-126) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 36-161 representing the extracellular domain of CTLA-4 of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins CD80 (B7-1) and CD86 (B7-2) are expressed on antigen presenting cells, bind the homologous T cell receptors CD28 and CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31% overall amino acid identity with CD28 and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counterreceptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | canine, bovine and porcine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 36-161 representing the extracellular domain of CTLA-4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 36-161 representing the extracellular domain of CTLA-4 of human origin |
| Entrez Gene | 1493 |
|---|
Application
Western blot analysis of CTLA-4 expression in Jurkat (A), ALL-S1L (B), U-698-M (C), U266 (D) and CCRF-CEM (E) whole cell lysates.
Application
Western blot analysis of human recombinant CTLA-4 fusion protein.| Application Notes | WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
