Application
Western blot analysis of N-type Ca++ CP α1B expression in SH-SY5Y (A), U-87 MG (B) and IMR-32 (C) whole cell lysates and rat brain tissue extract (D).Application
Western blot analysis of N-type Ca++ CP α1B expression in SH-SY5Y whole cell lysate.| Product Name | N-type Ca++ CP α1B Antibody (H-155) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1841-1995 mapping near the C-terminus of N-type calcium channel α1B of human origin |
| Modification Notes | N-type calcium channels are localized in high density presynaptic nerve N-type calcium channels are localized in high density presynaptic nerve terminals and are crucial elements in neuronal excitation-secretion coupling. Peripherally distributed N-type Ca++ channel plays a key role in cardiovascular regulation through autonomic nervous system. The high-voltage activated Ca++ channels that have been characterized biochemically are complexes of a pore-forming a-1 subunit; a transmembrane, disulfide-linked complex of a-2 and d subunits; an intracellular b subunit; and in some cases, a transmembrane g subunit. The a-1 subunit conducts N-type Ca++ currents, which initiate rapid synaptic transmission. In addition to mediating Ca++ entry to initiate transmitter release, N-type Ca++ channels are thought to interact directly with proteins of the synaptic vesicle docking and fusion machinery. The synaptic protein interaction sites in the intracellular loop II-III of subunit a-1B of N-type Ca++ channels bind to syntaxin, SNAP-25 and synaptotagmin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of N-type calcium channel α1B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1841-1995 mapping near the C-terminus of N-type calcium channel &alpha 1B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1841-1995 mapping near the C-terminus of N-type calcium channel α1B of human origin |
| Entrez Gene | 774 |
|---|
Application
Western blot analysis of N-type Ca++ CP α1B expression in SH-SY5Y (A), U-87 MG (B) and IMR-32 (C) whole cell lysates and rat brain tissue extract (D).
Application
Western blot analysis of N-type Ca++ CP α1B expression in SH-SY5Y whole cell lysate.| Application Notes | recommended for detection of N-type calcium channel α1B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
