Application
Western blot analysis of ACE expression in IB4 whole cell lysate.Application
Western blot analysis of ACE expression in mouse kidney tissue extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane localization.| Product Name | ACE Antibody (H-170) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-170 mapping at the N-terminus of ACE somatic isoform of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Angiotensin-converting enzyme (ACE) is a carboxy-terminal dipeptidyl exo-peptidase that converts Angiotensin I to the potent vasopressive hormone, Angiotensin II. There are two isoforms of ACE, the pulmonary ACEP and the testicular ACET. ACEP is a glycoprotein expressed in vascular endothelial cells of the lung, liver, adrenal cortex, pancreas, kidney and spleen. The ACET isoform is expressed exclusively in adult testis by developing sperm cells, specifically, late pachytene spermatocytes. Additionally, ACE inactivates bradykinin, a vasodepressor peptide, and is involved in fluid/electrolyte homeostasis. Although it bears significant sequence homology to ACE, ACE2 shows a more restricted pattern of expression. ACE is expressed ubiquitously throughout the vasculature while ACE2 is expressed only in cardiac, renal and testicular cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC-p:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-170 mapping at the N-terminus of ACE somatic isoform of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-170 mapping at the N-terminus of ACE somatic isoform of human origin |
| Entrez Gene | 1636 |
|---|
Application
Western blot analysis of ACE expression in IB4 whole cell lysate.
Application
Western blot analysis of ACE expression in mouse kidney tissue extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane localization.| Application Notes | WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC-p:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
