Application
Immunoper-oxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Fatty Acid Synthase expression in non-transfected: (A) and human Fatty Acid Synthase transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Fatty Acid Synthase expression in mouse liver tissue extract.Application
Western blot analysis of UGT1A expression in non-transfected: (A) and human Fatty Acid Synthase transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast cancer showing cytoplasmic staining of tumor cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed A549 cells showing cytoplasmic localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse lung tissue showing cytoplasmic localization.Application
Western blot analysis of Fatty Acid Synthase expression in c4 (A) and A549 (B) whole cell lysates.Application
Antibodies tested include Fatty Acid Synthase (C-20): (A) and Fatty Acid Synthase (H-300): (B).| Product Name | Fatty Acid Synthase Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against amino acids 2205-2504 of Fatty Acid Synthase of human origin |
| Modification Notes | Fatty acid biosynthesis is mediated by seven catalytic enzymes and an acyl carrier protein (ACP), to which various acyl intermediates are covalently attached. Fatty Acid Synthase (FAS) is the anabolic enzyme that contains the seven unique catalytic sites and mediates the conversion of acetyl-CoA and malonyl-CoA, in the presence of the cofactor NADPH, into long-chain saturated fatty acids, such as palmitate. Human Fatty Acid Synthase cDNA encodes a 2,504 amino acid protein. Catalytically active Fatty Acid Synthase is a homodimer. Human Fatty Acid Synthase mRNA is variably expressed with abundant levels present in brain, lung and liver. Fatty acid synthetic metabolism is abnormally elevated in tumor cells and may support cell growth or survival of malignant cancers. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Fatty Acid Synthase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 2205-2504 of Fatty Acid Synthase of human origin |
|---|---|
| Tissue Specificity | raised against amino acids 2205-2504 of Fatty Acid Synthase of human origin |
| Entrez Gene | 2194 |
|---|
Application
Immunoper-oxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Fatty Acid Synthase expression in non-transfected: (A) and human Fatty Acid Synthase transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Fatty Acid Synthase expression in mouse liver tissue extract.
Application
Western blot analysis of UGT1A expression in non-transfected: (A) and human Fatty Acid Synthase transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast cancer showing cytoplasmic staining of tumor cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed A549 cells showing cytoplasmic localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse lung tissue showing cytoplasmic localization.
Application
Western blot analysis of Fatty Acid Synthase expression in c4 (A) and A549 (B) whole cell lysates.
Application
Antibodies tested include Fatty Acid Synthase (C-20): (A) and Fatty Acid Synthase (H-300): (B).| Application Notes | recommended for detection of Fatty Acid Synthase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
