Application
Western blot analysis of VGF expression in NGF treated PC-12 whole cell lysate.| Product Name | VGF Antibody (H-65) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 159-223 mapping within an internal region of VGF of human origin |
| Modification Notes | Nerve growth factor (NGF) is a peptide that plays a key role in the differentiation and survival of neurons in the peripheral nervous system (PNS) and the central nervous system (CNS). VGF is a peptide synthesized and secreted by neurons and is upregulated by NGF in the PC12 cell line. VGF is widely expressed in both the PNS and CNS, but is especially abundant in the adult hypothalamus. VGF plays an essential role in how the brain regulates energy expenditure and body weight. Its expression is rapidly induced by injury, the circadian clock, and neuronal activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of VGF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 159-223 mapping within an internal region of VGF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 159-223 mapping within an internal region of VGF of human origin |
| Entrez Gene | 7425 |
|---|
Application
Western blot analysis of VGF expression in NGF treated PC-12 whole cell lysate.| Application Notes | recommended for detection of VGF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
