Application
Western blot analysis of DNA pol γ expression in non-transfected: (A) and human DNA pol γ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of DNA pol expression in non-transfected: (A) and human DNA pol transfected: (B) 293T whole cell lysates.Application
Western blot analysis of DNA pol γ expression in non-transfected 293T: (A) and human DNA pol γ transfected 293T: (B) whole cell lysates and HeLa nuclear extract (C).| Product Name | DNA pol γ Antibody (H-215) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. These exonucleases include the family of DNA polymerases (DNA pol). DNA pol å, ∫, ∂, and epsilon are involved in DNA replication and repair. DNA pol ∂ and DNA pol e are multi-subunit enzymes, with DNA pol ∂ consisting of two subunits: p125, which interacts with the sliding DNA clamp protein, PCNA, and p50. The nuclear-encoded DNA pol © is the only DNA polymerase required for the replication of the mitochondrial DNA. DNA pol zeta is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis. DNA pol theta is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB,IP,IF,ELISA |
| recommended for detection of DNA pol &gamma of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1025-1239 mapping at the C-terminus of DNA pol &gamma of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1025-1239 mapping at the C-terminus of DNA pol γ of human origin |
| Entrez Gene | 5428 |
|---|
Application
Western blot analysis of DNA pol γ expression in non-transfected: (A) and human DNA pol γ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of DNA pol expression in non-transfected: (A) and human DNA pol transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of DNA pol γ expression in non-transfected 293T: (A) and human DNA pol γ transfected 293T: (B) whole cell lysates and HeLa nuclear extract (C).| Application Notes | recommended for detection of DNA pol &gamma of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
