Application
Immunoperox-idase staining of formalin fixed, paraffin-embedded human esophagus tissue showing cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of Relaxin Receptor 1 expression in human stomach (A) and human heart (B) tissue extracts.Application
Western blot analysis of Relaxin Receptor 1 expression in THP-1 whole cell lysate.| Product Name | Relaxin Receptor 1 Antibody (H-160) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | G protein-coupled receptors (GPRs) are a protein family of transmembrane receptors that transmit an extracellular signal (ligand binding) into an intracellular signal (G protein activation). Relaxin Receptor 1, also known as Relaxin/insulin-like family peptide receptor 1, RXFP1, LGR7 or RXFPR1, is a leucine-rich repeat G protein-coupled receptor that binds Relaxins and INSL3 (insulin-like peptide 3). Expressed in brain, placenta, uterus, kidney, prostate, testis, adrenal, heart, ovary and skin, Relaxin Receptor 1 localizes to the cell membrane and contains ten LRR (leucine-rich) repeats and an LDL-receptor class A domain. Upon Relaxin or INSL3 binding to Relaxin Receptor 1, adenylate (A) cyclase is activated, leading to an increased intracellular concentration of cAMP. cAMP is a key intracellular regulator; it mediates the activities of numerous hormones, including ACTH, Glucagon and epinephrine, and plays an important role in modulating cellular activity. Due to alternative splicing events, two Relaxin Receptor 1 isoforms are expressed. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB,IP,IF,IHC,ELISA |
| recommended for detection of Relaxin Receptor 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including porcine | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 61-220 mapping within an N-terminal extracellular domain of Relaxin Receptor 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 61-220 mapping within an N-terminal extracellular domain of Relaxin Receptor 1 of human origin |
| Entrez Gene | 59350 |
|---|
Application
Immunoperox-idase staining of formalin fixed, paraffin-embedded human esophagus tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of Relaxin Receptor 1 expression in human stomach (A) and human heart (B) tissue extracts.
Application
Western blot analysis of Relaxin Receptor 1 expression in THP-1 whole cell lysate.| Application Notes | recommended for detection of Relaxin Receptor 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
