Application
Western blot analysis of splicing factor 1 expression in HeLa whole cell lysate.Application
Western blot analysis of splicing factor 1 expression in non-transfected 293T: (A) and human splicing factor 1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of splicing factor 1 expression in non-transfected: (A) and human splicing factor 1 transfected: (B) 293T whole cell lysates.| Product Name | splicing factor 1 Antibody (H-160) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Mammalian splicing factor 1 (designated SF1, zinc finger protein 162, ZFM1, CW17R and mammalian branch point binding protein [mBBP]) specifically recognizes the seven-nucleotide branch point sequence located at 3’ splice sites and participates in the assembly of early spliceosomal complexes (1,2). Splicing factor 1 functions as a transcriptional repressor and may control both proliferation and expression of pro-inflammatory gene products in smooth muscle cells (3). In addition, cytokine-induced downregulation of splicing factor 1 expression may contribute to the pathogenesis of hyperproliferative inflammatory diseases (3). The structure of splicing factor 1 contains a nuclear transport domain, a metal binding motif, and glutamine- and proline-rich regions (4). Human splicing factor 1 also exists as several different isoforms, H1-isoform and Bo-isoform, produced by alternative splicing events (5). The human splicing factor 1 gene is located on chromosome 11 close to the gene encoding Menin, the gene responsible for multiple endocrine neoplasia-type 1 (MEN1) (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB,IP,IF,ELISA,GS,ChIP |
| recommended for detection of splicing factor 1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-160 mapping at the N-terminus of splicing factor 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-160 mapping at the N-terminus of splicing factor 1 of human origin |
| Entrez Gene | 7536 |
|---|
Application
Western blot analysis of splicing factor 1 expression in HeLa whole cell lysate.
Application
Western blot analysis of splicing factor 1 expression in non-transfected 293T: (A) and human splicing factor 1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of splicing factor 1 expression in non-transfected: (A) and human splicing factor 1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of splicing factor 1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
