Application
Western blot analysis of Op18 expression in K-562 whole cell lysate.Application
Western blot analysis of Op18 expression in SK-MEL-28 whole cell lysate.| Product Name | Op18 Antibody (FL-149) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-149 of Op18 of human origin |
| Modification Notes | Op18 (for oncoprotein 18, also designated stathmin, prosolin or metablastin) is a conserved, tubulin-associated, intracellular phosphoprotein (1,2). Many different phosphorylated forms of Op18 are observed, and it is expressed as two different isoforms (2). Op18 is considered a critical regulator of microtubulin dynamics and is downregulated by p53 (1,3,4). It serves as a transducing protein, via phophorylation, for a variety of cell signaling pathways and involved in both mitosis and differentiation (5). Op18 is present in many cancers, including breast carcinomas, and is highly expressed in acute leukemias of different subtypes (6,7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Op18 and other stathmin family members including stathmin-like protein RB3 and SCG10-like protein of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-149 of Op18 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-149 of Op18 of human origin |
| Entrez Gene | 3925 |
|---|
Application
Western blot analysis of Op18 expression in K-562 whole cell lysate.
Application
Western blot analysis of Op18 expression in SK-MEL-28 whole cell lysate.| Application Notes | recommended for detection of Op18 and other stathmin family members including stathmin-like protein RB3 and SCG10-like protein of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
