Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human hippocampus tissue showing cytoplasmic staining of neuronal cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of PP2B-Aα expression in non-transfected: (A) and human PP2B-Aα transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PP2B-A expression in mouse brain extract.| Product Name | PP2B-A Antibody (H-209) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 312-521 mapping at the C-terminus of PP2B-Aα of human origin |
| Modification Notes | In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4), is a putative member of a novel PP family. The PP2B family comprises subfamily members PP2B-Aå, PP2B-A∫ and PP2B-A©. Two additional regulatory subunits been identified, designated PP2B-B1 and PP2B-B2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of all PP2B-A isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 312-521 mapping at the C-terminus of PP2B-A&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 312-521 mapping at the C-terminus of PP2B-Aα of human origin |
| Entrez Gene | 5530 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human hippocampus tissue showing cytoplasmic staining of neuronal cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of PP2B-Aα expression in non-transfected: (A) and human PP2B-Aα transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PP2B-A expression in mouse brain extract.| Application Notes | recommended for detection of all PP2B-A isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
