Application
Western blot analysis of TGFβ RII expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of HIS-tagged human recombinant TGFβ RII.Application
Sections probed with TGFβ RII (C-16): (A) and TGFβ RII (L-21): (B). Kindly provided by Leslie Gold.| Product Name | TGFβ RII Antibody (L-21) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within a cytoplasmic domain of TGFβ RII of human origin |
| Modification Notes | A total of three members of the TGF∫ family, TGF∫1, TGF∫2 and TGF∫3, have been identified in mammals. Each is synthesized as a latent precursor that is subsequently cleaved forming the 112 amino acid growth factor which becomes active upon dimerization. TGF∫s mediate their activity by high affinity binding to the type II receptor (TGF∫ RII) transmembrane protein with a cytoplasmic serine-threonine kinase domain. TGF∫ RII (TGF-beta receptor type-2), also known as TGFBR2, is a 567 amino acid single-pass type I membrane protein that contains one protein kinase domain and is a member of the protein kinase superfamily, TKL Ser/Thr protein kinase family and TGFB receptor subfamily. For signaling growth inhibition and early gene responses, TGF∫ RII requires both its kinase activity and association with a TGF∫-binding protein, designated the type I receptor. TGF∫ RII exists as two alternatively spliced isoforms that are encoded by a gene that maps to human chromosome 3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TGFβ RII p70 of mouse, rat, human and ovine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within a cytoplasmic domain of TGF&beta RII of human origin |
|---|---|
| Tissue Specificity | epitope mapping within a cytoplasmic domain of TGFβ RII of human origin |
| Entrez Gene | 7048 |
|---|
Application
Western blot analysis of TGFβ RII expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of HIS-tagged human recombinant TGFβ RII.
Application
Sections probed with TGFβ RII (C-16): (A) and TGFβ RII (L-21): (B). Kindly provided by Leslie Gold.| Application Notes | recommended for detection of TGFβ RII p70 of mouse, rat, human and ovine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
