Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of subset of cells in Islets of Langerhans and glandular cells.Application
Western blot analysis of Keap1 expression in Hep G2 whole cell lysate.Application
Western blot analysis of Keap1 expression in non-transfected: (A) and human Keap1 transfected: (B) 293T whole cell lysates.| Product Name | Keap1 Antibody (H-190) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Keap1 (kelch-like ECH-associated protein 1, INrf2, KLHL19) is a stress sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex that negatively regulates Nrf2 (NF-E2-related factor 2). Steady state levels of proteins are under the influence of the ubiquitin pathway, which consists of ubiquitin activation (E1), conjugation (E2) and ligation (E3). Keap1 assembles into an E3 ubiquitin ligase complex with Cul3 and Rbx1 and targets lysine residues in the N-terminal Neh2 domain of Nrf2 for ubiquitin conjugation. The Keap1-Nrf2 system mediates cytoprotective gene expression in response to oxidative and/or electrophilic stresses. Keap1 constitutively suppresses Nrf2 activity under unstressed conditions, oxidants or electrophiles provoke the repression of Keap1 activity, inducing Nrf2 activation. Cys 273 and Cys 288 residues of Keap1 are required for suppressing Nrf2 nuclear accumulation. Keap1 sequesters Nrf2 in the cytoplasm through an active Crm1/exportin-dependent nuclear export mechanism. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB,IP,IF,IHC,ELISA |
| recommended for detection of Keap1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 181-370 mapping within an internal region of Keap1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 181-370 mapping within an internal region of Keap1 of human origin |
| Entrez Gene | 9817 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of subset of cells in Islets of Langerhans and glandular cells.
Application
Western blot analysis of Keap1 expression in Hep G2 whole cell lysate.
Application
Western blot analysis of Keap1 expression in non-transfected: (A) and human Keap1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Keap1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
