| Product Name | βPIX Antibody (H-115) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 346-460 mapping to an internal region of βPIX of human origin |
| Modification Notes | The serine/threonine kinase, p21 activated kinase (PAK), is a downstream effector of the small GTPases Cdc42 and Rac (1-3). PAK associates with Nck, the p85 and p110 subunits of PI3-kinase, and PIX (PAK-interacting exchange factor) in a focal complex (1). The binding of PIX is necessary for the localization and activation of PAK in the Cdc42 to Rac signaling pathway, and this binding occurs through the high affinity of the N-terminal SH3 domain of PIX for a conserved proline rich PAK sequence (1,2,4,5). PIX exists as two isoforms, å and ∫ and both are highly expressed in heart, muscle, and thymus tissues of human and rat (1,2). åPIX is phosphorylated via PDGF and EphB2 receptor signaling pathways or through association with PI3-kinase (1). The åPIX isoform predominantly acts as a guanine nucleotide exchange factor (GEF) on Rac, which may mediate lamellipodia formation (1,3,4,6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of βPIX of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 346-460 mapping to an internal region of &beta PIX of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 346-460 mapping to an internal region of βPIX of human origin |
| Entrez Gene | 8874 |
|---|
| Application Notes | recommended for detection of βPIX of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
