Application
Western blot analysis of PAK4 expression in non-transfected: (A) and human PAK4 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PAK4 expression in rat testis tissue extract.Application
Western blot analysis of PAK4 expression in non-transfected: (A) and mouse PAK4 transfected: (B) 293T whole cell lysates and rat testis tissue extract (C).Application
Western blot analysis of PAK4 expression in non-transfected: (A) and human PAK4 transfected: (B) 293 whole cell lysates.| Product Name | PAK4 Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 101-240 mapping near the N-terminus of PAK4 of human origin |
| Modification Notes | Three recently identified isoforms of serine/threonine kinases, designated åPAK p68, ∫PAK p65 and ©PAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase STE20, involved in pheromone signaling. The å, ∫ and ©PAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho. PAK4 is highly expressed in prostate, testis and colon. PAK4 interacts tightly with GTP-bound but not GDP-bound CDC42 and weakly with RAC. PAK4 phosphorylates and autophosphorylates and also activates the JNK pathway. Coexpression of PAK4 and activated CDC42 induces the sustained formation of actin-enriched filopodia protrusions and causes PAK4 to colocalize with polymerized actin clusters and with beta coat protein in the Golgi. The gene which encodes PAK4 maps to human chromosome 19. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PAK4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 101-240 mapping near the N-terminus of PAK4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 101-240 mapping near the N-terminus of PAK4 of human origin |
| Entrez Gene | 10298 |
|---|
Application
Western blot analysis of PAK4 expression in non-transfected: (A) and human PAK4 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PAK4 expression in rat testis tissue extract.
Application
Western blot analysis of PAK4 expression in non-transfected: (A) and mouse PAK4 transfected: (B) 293T whole cell lysates and rat testis tissue extract (C).
Application
Western blot analysis of PAK4 expression in non-transfected: (A) and human PAK4 transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of PAK4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
