Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse ovary tissue showing nuclear and cytoplasmic localization.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.Application
Western blot analysis of Smad expression in NIH/3T3 (A), Mv 1 Lu (B) and ZR-75-1 (C) whole cell lysates.| Product Name | Smad Antibody (H-465) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-465 representing full length Smad1 of human origin |
| Modification Notes | Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGF∫/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGF∫ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGF∫ signaling by interfering with TGF∫-mediated phosphorylation of other Smad family members. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Smad1, Smad2, Smad3, Smad5 and Smad8 of mouse, rat, human, and, to a lesser extent, mink origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-465 representing full length Smad1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-465 representing full length Smad1 of human origin |
| Entrez Gene | 4086 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse ovary tissue showing nuclear and cytoplasmic localization.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
Application
Western blot analysis of Smad expression in NIH/3T3 (A), Mv 1 Lu (B) and ZR-75-1 (C) whole cell lysates.| Application Notes | recommended for detection of Smad1, Smad2, Smad3, Smad5 and Smad8 of mouse, rat, human, and, to a lesser extent, mink origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
