Application
Western blot analysis of NFκB p65 expression in HeLa (A) and MIA PaCa-2 (B) whole cell lysates.Application
Western blot analysis of NFκB p65 expression in SK-BR-3 whole cell lysate.Application
Western blot analysis of NFκB p65 expression in non-transfected: (A) and mouse NFκB p65 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NFκB p65 expression in non-transfected: (A) and human NFκB p65 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NFκB p65 expression in non-transfected 293T: (A), human NFκB p65 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): , p300 (C-20): 585, p300 (N-15): 584, p300 (H-272): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , Bcl-3 (C-14): , Bcl-3 (H-146): , PCAF (C-16): , PCAF (H-369): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.Application
IP's permorned without (A) and with (B) primary antibody. Antibodies tested include NFκB p65 (A): , RelB (C-19): and p300 (N-15): . Data kindly provided by A. Hoffmann.Application
Antibodies tested included NFκB p65 (A): , ATF-2 (N-96): , GCN5 (N-18): , Pol II (H-224): , TFIID (TBP)(SI-1): and CBP (A-22): . Data kindly provided by G. Mosialos.Application
Western blot analysis of NFκB p65 expression in extracts of human A-431 (A) and K-562 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.Application
Cells were immunostained with NFκB p65 (A): and visualized with biotinylated goat anti-rabbit IgG and fluorescein isothiocyanate-conjugated avidin. Kindly provided by Sree Devi Menon.| Product Name | NFκB p65 Antibody (A) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within the N-terminus of NFκB p65 of human origin |
| Modification Notes | Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NF˚B (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp ˚B sequence in the immunoglobulin k light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFkB is activated and NFkB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of NFκB p65 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within the N-terminus of NF&kappa B p65 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within the N-terminus of NFκB p65 of human origin |
| Entrez Gene | 5970 |
|---|
Application
Western blot analysis of NFκB p65 expression in HeLa (A) and MIA PaCa-2 (B) whole cell lysates.
Application
Western blot analysis of NFκB p65 expression in SK-BR-3 whole cell lysate.
Application
Western blot analysis of NFκB p65 expression in non-transfected: (A) and mouse NFκB p65 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NFκB p65 expression in non-transfected: (A) and human NFκB p65 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NFκB p65 expression in non-transfected 293T: (A), human NFκB p65 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): , p300 (C-20): 585, p300 (N-15): 584, p300 (H-272): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , Bcl-3 (C-14): , Bcl-3 (H-146): , PCAF (C-16): , PCAF (H-369): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
Application
IP's permorned without (A) and with (B) primary antibody. Antibodies tested include NFκB p65 (A): , RelB (C-19): and p300 (N-15): . Data kindly provided by A. Hoffmann.
Application
Antibodies tested included NFκB p65 (A): , ATF-2 (N-96): , GCN5 (N-18): , Pol II (H-224): , TFIID (TBP)(SI-1): and CBP (A-22): . Data kindly provided by G. Mosialos.
Application
Western blot analysis of NFκB p65 expression in extracts of human A-431 (A) and K-562 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.
Application
Cells were immunostained with NFκB p65 (A): and visualized with biotinylated goat anti-rabbit IgG and fluorescein isothiocyanate-conjugated avidin. Kindly provided by Sree Devi Menon.| Application Notes | recommended for detection of NFκB p65 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
