WB
(A, G) Western blotting and quantitative analysis of the SLIT3 expression (n = 3).WB
(A, G) Western blotting and quantitative analysis of the SLIT3 expression (n = 3).IF
(B, H) Immunofluorescence staining and quantitative analysis of SLIT3 (green) in LEPR+ BMSCs (n = 3). Nuclei, DAPI (blue). Scale bar = 100 μm.IF
(B, H) Immunofluorescence staining and quantitative analysis of SLIT3 (green) in LEPR+ BMSCs (n = 3). Nuclei, DAPI (blue). Scale bar = 100 μm.| Product Name | Slit3 Antibody (H-54) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Secreted leucine-rich repeat-containing proteins 1-3 (Slit1-3) are secreted glycoproteins that influence axonal guidance and mediate normal neural development by acting as high-affinity signaling ligands for the repulsive guidance receptor, Roundabout (Robo) (1, 2). Within the developing central nervous system (CNS) of different vertebrate systems, Slit proteins are expressed in equivalent regions, suggesting a conserved function among vertebrate homologs (3,4). Slit is expressed in the midline of the central nervous system in both vertebrates and invertebrates, where it functions as a regulatory factor of mesodermal cell movement during gastrulation (5). Slit2 is a short range inhibitory guidance cue for retinal ganglion cell (RGC) axons that may mediate spatial progression of RGCs (6,7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| recommended for detection of Slit3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 231-284 mapping near the N-terminus of Slit3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 231-284 mapping near the N-terminus of Slit3 of human origin |
| Entrez Gene | 6586 |
|---|
WB
(A, G) Western blotting and quantitative analysis of the SLIT3 expression (n = 3).
WB
(A, G) Western blotting and quantitative analysis of the SLIT3 expression (n = 3).
IF
(B, H) Immunofluorescence staining and quantitative analysis of SLIT3 (green) in LEPR+ BMSCs (n = 3). Nuclei, DAPI (blue). Scale bar = 100 μm.
IF
(B, H) Immunofluorescence staining and quantitative analysis of SLIT3 (green) in LEPR+ BMSCs (n = 3). Nuclei, DAPI (blue). Scale bar = 100 μm.| Application Notes | recommended for detection of Slit3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
