Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing nuclear and cytoplasmic staining of cells in fallopian tubes.Application
Western blot analysis of c-IAP1 expression in Jurkat (A) and MOLT-4 (B) whole cell lysates.| Product Name | c-IAP1 Antibody (H-83) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 111-193 mapping within an internal region of c-IAP1 (inhibitor of apoptosis protein 1) of human origin |
| Modification Notes | The baculovirus protein p35 inhibits virally induced apoptosis of invertebrate and mammalian cells and may function to impair the clearing of virally infected cells by the immune system of the host. This is accomplished at least in part by the ability of p35 to block both TNF- and FAS-mediated apoptosis through the inhibition of the ICE family of serine proteases. Three mammalian homologs of baculovirus p35, designated MIHA (mammalian IAP homolog A), MIHB and MIHC have been described. These three mammalian inhibitor of apoptosis proteins (IAPs) are designated XIAP, c-IAP1 and c-IAP2, respectively. XIAP, c-IAP1 and c-IAP2 share an N-terminal baculovirus IAP repeat (BIR) motif and a C-terminal RING finger. Although c-IAP1 and c-IAP2 do not directly associate with the TNF receptor (TNF-R), they efficiently block TNF-mediated apoptosis through their interaction with the downstream TNF-R effectors, TRAF1 and TRAF2. The interaction between the TRAF1/TRAF2 heterocomplexes and c-IAPs is dependent on a functional BIR motif. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of c-IAP1 by human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 111-193 mapping within an internal region of c-IAP1 (inhibitor of apoptosis protein 1) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 111-193 mapping within an internal region of c-IAP1 (inhibitor of apoptosis protein 1) of human origin |
| Entrez Gene | 329 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing nuclear and cytoplasmic staining of cells in fallopian tubes.
Application
Western blot analysis of c-IAP1 expression in Jurkat (A) and MOLT-4 (B) whole cell lysates.| Application Notes | recommended for detection of c-IAP1 by human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
