Application
Western blot analysis of NOXA expression in non-transfected 293T: (A), human NOXA transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of NOXA expression in Jurkat (A), U-937 (B), RAW 264.7 (C) and HUT 78 (D) whole cell lysates.| Product Name | NOXA Antibody (FL-54) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-54 representing full length NOXA of human origin |
| Modification Notes | Members of the Bcl-2 family of proteins interact to regulate programmed cell death (apoptosis) under a broad range of physiological conditions. Bcl-2, Bcl-xL, and several related proteins inhibit apoptosis, whereas other members of the Bcl-2 family, such as Bax and Bak, enhance cell death. NOXA, a pro-apoptotic member of the Bcl-2 family, contains the Bcl-2 homology 3 (BH3) region, but does not contain other BH domains. Murine cells constitutively express NOXA mRNA in small amounts in various organs; X-ray irradiation increases NOXA mRNA and protein expression levels. In human cells, NOXA, alternatively designated PMA-induced protein 1 or APR, displays high expression in the adult T cell leukemia cell line IKD, where it may function as an immediate-early-response gene. The NOXA protein selectively localizes to mitochondria. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NOXA of human and, to a lesser extent, mouse origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-54 representing full length NOXA of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-54 representing full length NOXA of human origin |
| Entrez Gene | 5366 |
|---|
Application
Western blot analysis of NOXA expression in non-transfected 293T: (A), human NOXA transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of NOXA expression in Jurkat (A), U-937 (B), RAW 264.7 (C) and HUT 78 (D) whole cell lysates.| Application Notes | recommended for detection of NOXA of human and, to a lesser extent, mouse origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
