Application
Western blot analysis of Dab2 phosphorylation in untreated (A) and lambda protein phosphatase treated HeLa whole cell lysates (B).Application
Western blot analysis of Dab2 phosphorylation in non-transfected: (A) and human Dab2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Dab2 phosphorylation in non-transfected 293T: (A), mouse Dab2 transfected 293T: (B) and Y79 (C) whole cell lysates.| Product Name | p-Dab2 Antibody (Ser 24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 24 of Dab2 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Dab2 (also designated DOC-2) is a mitogen-responsive phosphoprotein that binds the SH3 domain of Grb2, and it is thought to be a negative regulator of growth. Dab2 Ser24 phosphorylation promotes membrane translocation of Dab2 and its subsequent interaction with integrin beta3, thereby defining a mechanism for Dab2 in regulating CD61 activation and inside-out signaling. Dab2 Ser24 phosphorylation is also involved in the negative regulation of CD61-induced T cell factor (TCF) transcriptional activity. Dab2 elicits distinct regulatory mechanisms in CD61 and -catenin/plakoglobin signaling in a Ser24 phosphorylation-dependent and -independent manner, respectively. Dab1, a homolog of the Drosophila Disabled protein, is an adaptor protein involved in neural development. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ser 24 phosphorylated Dab2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 24 of Dab2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 24 of Dab2 of human origin |
| Entrez Gene | 1601 |
|---|
Application
Western blot analysis of Dab2 phosphorylation in untreated (A) and lambda protein phosphatase treated HeLa whole cell lysates (B).
Application
Western blot analysis of Dab2 phosphorylation in non-transfected: (A) and human Dab2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Dab2 phosphorylation in non-transfected 293T: (A), mouse Dab2 transfected 293T: (B) and Y79 (C) whole cell lysates.| Application Notes | recommended for detection of Ser 24 phosphorylated Dab2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
