Application
Western blot analysis of CD67 expression in human platelet whole cell lysate (A) and HL-60 (B), HEL 92.1.7 (C) and CCRF-CEM (D) whole cell lysates.| Product Name | CD67 Antibody (H-75) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 71-145 mapping within an internal region of CD67 of human origin |
| Modification Notes | Carcinoembryonic antigen-related cell adhesion molecule 8, also designated CD67, CD66b or nonspecific cross-reacting antigen (NCA-95), belongs to the human carcinoembryonic antigen (CEA) family. The CD67 antigen is encoded by the CEACAM8 (CGM6) gene, which is exclusively expressed in neutrophils and eosinophils. In neutrophils, the CEACAM8 gene is primarily detected in the secondary granules within the cytoplasm, but it can also be found in lower amounts on the plasma membrane. The amount of CD67 on the plasma membrane is up-regulated upon granulocyte activation. CD67 has been located on the surface of neutrophilic and eosinophilic granulocytes at late stages of differentiation. It exhibits heterophilic cell adhesion properties with CD66c, which is coexpressed with CD67 in granulocytes. CD67, which is attached to the membrane by a GPI-anchor, is expressed in leukocytes of chronic myeloid leukemia patients and bone marrow and in granulocytes in the spleen, thymus and lungs. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD67 and, to a lesser extent, CD66a, CD66c, CD66d and CD66e of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 71-145 mapping within an internal region of CD67 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 71-145 mapping within an internal region of CD67 of human origin |
| Entrez Gene | 1088 |
|---|
Application
Western blot analysis of CD67 expression in human platelet whole cell lysate (A) and HL-60 (B), HEL 92.1.7 (C) and CCRF-CEM (D) whole cell lysates.| Application Notes | recommended for detection of CD67 and, to a lesser extent, CD66a, CD66c, CD66d and CD66e of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
