Application
Western blot analysis of CRABP-I expression in non-transfected: (A) and human CRABP-I transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CRABP-I expression in non-transfected: (A) and human CRABP-I transfected: (B) 293T whole cell lysates.| Product Name | CRABP-I/II Antibody (H-105) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 26-130 of CRABP-I of human origin |
| Modification Notes | The cellular retinoic acid-binding protein (CRABP)-I and a related isoform, CRABP-II, bind retinoic acid (RA), an important regulator of cell growth and differentiation in fetal and adult tissues (1,2). These CRABP proteins mediate the downstream effects of RA in distinct ways (3). CRABP-I negatively regulates the activity of RA by enhancing the production of RA-metabolizing enzymes and increasing the rate at which RA is degraded (4). CRABP-II enhances the effects of RA by directly interacting with RA receptors (RAR) and, in turn, promoting the formation of RAR-RA complexes and stimulating RA-mediated gene transcription (5). Both CRABP-I and CRABP-II are expressed in the embryo, and CRABP-I is ubiquitously expressed in various adult tissues (6). The expression of CRABP-II is elevated in cells that synthesize relatively large amounts of RA, and it is also predominantly expressed in skin, uterus, ovary and in the choroid plexus (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CRABP-I and II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 26-130 of CRABP-I of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 26-130 of CRABP-I of human origin |
| Entrez Gene | 1381 |
|---|
Application
Western blot analysis of CRABP-I expression in non-transfected: (A) and human CRABP-I transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CRABP-I expression in non-transfected: (A) and human CRABP-I transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of CRABP-I and II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
