Application
Western blot analysis of GM130 expression in non-transfected 293T: (A), mouse GM130 transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of GM130 expression in rat liver tissue extract.| Product Name | GM130 Antibody (H-65) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 191-255 mapping within an internal region of GM130 of human origin |
| Modification Notes | The docking of transport vesicles to their target membrane is mediated by p115. GM130, a cis-Golgi matrix protein, interacts specifically with p115 and provides a membrane docking site. Both GM130 and p115 are involved in vesicle tethering to Golgi membranes. The amino-terminus of GM130 binds to p115, whereas the carboxy-terminus binds to Golgi membranes. Both Giantin and GM130 compete for binding to p115. Thus, p115-Giantin and p115-GM130 interactions might mediate independent membrane tethering events. Transport from the ER to the cis/medial Golgi compartments requires the action of p115, GM130 and Giantin via a sequential rather than a coordinate mechanism. Mitotic phosphorylation of GM130 at Serine 25 is mediated by Cdc2, prevents binding to p115 and is directly involved in mitotic Golgi fragmentation. GM130 is phosphorylated in prophase as the Golgi complex starts to break down, and remains phosphorylated in metaphase and anaphase. In telophase, GM130 is dephosphorylated by PP2A as the Golgi fragments start to reassemble. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GM130 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 191-255 mapping within an internal region of GM130 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 191-255 mapping within an internal region of GM130 of human origin |
| Entrez Gene | 2801 |
|---|
Application
Western blot analysis of GM130 expression in non-transfected 293T: (A), mouse GM130 transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of GM130 expression in rat liver tissue extract.| Application Notes | recommended for detection of GM130 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
