Application
Western blot analysis of fetuin-A expression in non-transfected: (A) and mouse fetuin-A transfected: (B) 293T whole cell lysates.Application
Western blot analysis of fetuin-A expression in Hep G2 (A) and human PBL (B) whole cell lysates.| Product Name | fetuin-A Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 68-367 of fetuin-A of human origin |
| Modification Notes | Fetuin (also designated a-2-z-globulin or a-2-HS-glycoprotein) is a secreted plasma protein that is expressed in hepatocytes, monocyte/ macrophages and in bone and is down-regulated during injury and inflammation (1). Fetuin preferentially binds to and carries calcium and barium ions in the blood, where it is thought to mediate serum calcium homeostasis and mineralization, and to potentially participate in the transport of bioactive molecules (2,3). Additionally, fetuin has been shown to function as an acute phase anti-inflammatory mediator that is critical to regulating the innate immune response following tissue injury (4). During inflammation, circulating fetuin levels substantially decrease as fetuin becomes associated with the membranes of macrophages (5). This membrane associated form of fetuin acts as an opsonic participant by potentiating the entry of cationic small molecules into the activated macrophage, which in turn facilitates macrophage-deactivating mechanisms (6). Biologically active fetuin is derived from a precursor protein that is cleaved at the amino terminus to generate two chains held together by a single disulfide bond (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of fetuin-A of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 68-367 of fetuin-A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 68-367 of fetuin-A of human origin |
| Entrez Gene | 197 |
|---|
Application
Western blot analysis of fetuin-A expression in non-transfected: (A) and mouse fetuin-A transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of fetuin-A expression in Hep G2 (A) and human PBL (B) whole cell lysates.| Application Notes | recommended for detection of fetuin-A of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
