Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic and nuclear staining of glandular cells.Application
Western blot analysis of POD-1 expression in non-transfected: (A) and human POD-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of POD-1 expression in non-transfected: (A) and mouse POD-1 transfected: (B) 293T whole cell lysates.| Product Name | POD-1 Antibody (H-75) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 6-80 mapping near the N-terminus of POD-1 of human origin |
| Modification Notes | The basic helix-loop-helix (bHLH) class of transcription factors govern cell fate determination by controlling a variety of cellular differentiation processes. POD-1 (podocyte-expressed 1, also designated capsulin or epicardin) is a nuclear bHLH protein that is involved in the specification of select mesodermal cell populations associated with heart, cranial skeletal muscle, gut and urogenital system. POD-1 is selectively expressed in mesenchymal cells at sites of epithelial-mesenchymal interaction in the kidney, lung, intestine, pancreas and the epicardium, which gives rise to the coronary arteries. This epithelial-mesenchymal interaction is involved in the formation of numerous internal organs. POD-1 is also expressed in the mesothelium that gives rise to the spleen and in cells that give rise to smooth muscle. In addition to its role in kidney morphogenesis and spleen organogenesis, POD-1 may play a role in the development and sex determination of the mammalian gonad. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of POD-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 6-80 mapping near the N-terminus of POD-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 6-80 mapping near the N-terminus of POD-1 of human origin |
| Entrez Gene | 6943 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic and nuclear staining of glandular cells.
Application
Western blot analysis of POD-1 expression in non-transfected: (A) and human POD-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of POD-1 expression in non-transfected: (A) and mouse POD-1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of POD-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
