Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.| Product Name | HB-EGF Antibody (H-88) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 121-208 of mature HB-EGF of human origin |
| Modification Notes | Heparin binding epidermal-like growth factor (HB-EGF), a member of the EGF family of mitogens, binds to the EGF receptor (EGFR) and to heparin sulfate proteoglycans on the cell surface. HB-EGF was originally isolated from medium conditioned by the growth of the human histocytic lymphoma cell U-937 on the basis of its heparin-binding ability and its mitogenic activity for Balb-3T3 fibroblasts. The HB-EGF gene encodes a 208 amino acid precursor containing a signal peptide and transmembrane domain. Mature HB-EGF is a soluble protein 86 amino acids in length and results from the enzymatic cleavage of the membrane bound precursor. The membrane-bound form of HB-EGF has been identified as the diphtheria toxin receptor. Preincubation of Vero cells with phorbol 12-myristate 13-acetate (PMA) induces the proteolytic cleavage of HB-EGF outside the membrane anchor. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HB-EGF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 121-208 of mature HB-EGF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 121-208 of mature HB-EGF of human origin |
| Entrez Gene | 1839 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.| Application Notes | recommended for detection of HB-EGF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
