Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear staining of glandular cells.Application
Western blot analysis of NELF-A expression in non-transfected: (A) and human NELF-A transfected: (B) 293T whole cell lysates and DU 145 nuclear extract (C).Application
Western blot analysis of NELF-A expression in non-transfected: (A) and human NELF-A transfected: (B) 293T whole cell lysates and DU 145 nuclear extract (C).Application
Western blot analysis of NELF-A expression in non-transfected: (A) and mouse NELF-A transfected: (B) 293T whole cell lysates and DU 145 nuclear extract (C).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymus tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Product Name | NELF-A Antibody (H-240) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 92-300 mapping near the N-terminus of NELF-A of human origin |
| Modification Notes | NELF-A, for negative elongation factor A, is a protein factor required for DRB-sensitive transcription. NELF-A is one of the five components of the multisubunit NELF complex that cooperates with DSIF to repress RNA polymerase II elongation. Control of transcription elongation requires a complex interplay between positive transcription elongation factor b (P-TEFb) and negative transcription elongation factors, DSIF and NELF. DSIF and NELF act as negative transcription elongation factors by increasing the time the polymerase spends at pause sites. DSIF/NELF inhibition of transcription is prevented by P-TEFb in cooperation with FACT. NELF-A is also known as WHSC2 (Wolf-Hirschhorn syndrome candidate 2). Wolf-Hirschhorn syndrome is a multiple malformation syndrome characterized by mental and developmental defects resulting from a hemizygous deletion of the distal short arm of chromosome 4 (4p16.3). The human NELF-A gene maps to chromosome 4p16.3 and encodes a 528 amino acid protein that is expressed in endothelial cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of NELF-A isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 92-300 mapping near the N-terminus of NELF-A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 92-300 mapping near the N-terminus of NELF-A of human origin |
| Entrez Gene | 7469 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear staining of glandular cells.
Application
Western blot analysis of NELF-A expression in non-transfected: (A) and human NELF-A transfected: (B) 293T whole cell lysates and DU 145 nuclear extract (C).
Application
Western blot analysis of NELF-A expression in non-transfected: (A) and human NELF-A transfected: (B) 293T whole cell lysates and DU 145 nuclear extract (C).
Application
Western blot analysis of NELF-A expression in non-transfected: (A) and mouse NELF-A transfected: (B) 293T whole cell lysates and DU 145 nuclear extract (C).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymus tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Application Notes | recommended for detection of NELF-A isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
