Application
Western blot analysis of c-Yes expression in non-transfected: (A) and mouse c-Yes transfected: (B) 293T whole cell lysates.Application
Western blot analysis of c-Yes expression in human platelet whole cell lysate.| Product Name | c-Yes Antibody (H-95) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-95 mapping at the N-terminus of c-Yes of human origin |
| Modification Notes | Src is the human homolog of the v-Src gene of the Rous sarcoma virus, also known as avian sarcoma virus or ASV. Src is the first proto-oncogenic non-receptor tyrosine kinase characterized in human. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src-family kinases transduce signals that control a variety of cellular processes, including proliferation, differentiation, motility and adhesion. Src-family kinases contain an amino terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Human c-Yes is the cellular homolog of the Yamaguchi sarcoma virus oncogene, Yes1. The human c-Yes gene maps to chromosome 18p11.31-p11.21 and encodes a 543 amino acid protein. c-Src and c-Yes kinases are more than 80% homologous outside of unique amino-termini. Their respective SH3 and SH2 domains are capable of directing specificity in substrate binding. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of c-Yes p61 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-95 mapping at the N-terminus of c-Yes of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-95 mapping at the N-terminus of c-Yes of human origin |
| Entrez Gene | 7525 |
|---|
Application
Western blot analysis of c-Yes expression in non-transfected: (A) and mouse c-Yes transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of c-Yes expression in human platelet whole cell lysate.| Application Notes | recommended for detection of c-Yes p61 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
