Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic and extracellular staining.Application
Western blot analysis of pro-MCH expression in JAR (A) and HeLa (B) whole cell lysates.| Product Name | pro-MCH Antibody (H-143) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 23-165 of pro-MCH precursor of human origin |
| Modification Notes | Melanin-concentrating hormone (MCH) is a 19 amino acid cyclic neuropeptide derived from a 165 amino acid pro-MCH precursor. In addition to the hormone, the pro-MCH precursor contains a 144 amino acid mature MCH as well as a 12 amino acid neuropeptide glycine-glutamic acid (NGE) and a 19 amino acid neuropeptide glutamic acid-isoleucine (NEI). Mainly expressed in the hypothalamus, the melanin-concentrating hormone modulates feeding behavior, aggression, anxiety, arousal and reproductive function in mammals by controlling the release of luteinizing hormone (LH). The melanin-concentrating hormone receptor (MCHR, also designated SLC-1) is a glycosylated G protein-coupled receptor. MCHR mediates the effects of MCH through Gåi and/or Gåq signaling and is expressed in several regions of the brain, including the cerebral cortex, amygdala, thalamus and hypothalamus. MCH and MCHR have also been implicated in stimulating leptin expression and secretion in adipocytes, suggesting that the melanin-concentrating hormone and its receptor may be potential targets for modulating obesity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of mature MCH, pro-MCH, Neuropeptide G-E, Neuropeptide E-I and melanin-concentrating hormone of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 23-165 of pro-MCH precursor of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 23-165 of pro-MCH precursor of human origin |
| Entrez Gene | 5367 |
|---|
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic and extracellular staining.
Application
Western blot analysis of pro-MCH expression in JAR (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of mature MCH, pro-MCH, Neuropeptide G-E, Neuropeptide E-I and melanin-concentrating hormone of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
