Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoskeletal staining.| Product Name | Plectin Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 4605-4684 mapping at the C-terminus of Plectin of human origin |
| Modification Notes | Plectin is an abundant cytoskeletal protein that is involved in cytoplasm stabilization. Plectin has been shown to crosslink intermediate filaments to microtubules and microfilaments, and to anchor intermediate filaments to the plasma and nuclear membranes. Plectin binds both Lamin B and vimentin, and this binding is regulated by a variety of protein kinases. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions. Plectin is also a substrate for p34cdc2 kinase. Several alternative splice isoforms of plectin are known to exist. Mutations in human plectin are known to cause epidermolysis bullosa simplex with muscular dystrophy (EBS-MD). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Plectin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 4605-4684 mapping at the C-terminus of Plectin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 4605-4684 mapping at the C-terminus of Plectin of human origin |
| Entrez Gene | 5339 |
|---|
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoskeletal staining.| Application Notes | recommended for detection of Plectin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
