Application
Western blot analysis of PEA-15 expression in non-transfected: (A) and mouse PEA-15 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PEA-15 expression in U-87 MG whole cell lysate.Application
Immunofluorescence staining of normal mouse kidney frozen section showing cytoplasmic staining.Application
Western blot analysis of human recombinant PEA-15 fusion protein.| Product Name | PEA-15 Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 51-130 mapping at the C-terminus of PEA-15 of human origin |
| Modification Notes | PEA-15 (Phosphoprotein Enriched in Astrocytes) exists in an non-phosphorylated form (N), and two phosphorylated forms, Pa and Pb. PEA-15 is an endogenous substrate for PKC, which mediates the transition from Pa to Pb. The level of PEA-15 phosphorylation changes upon depolymerization or stabilization of tubulins, indicating that PEA-15 colocalizes with microtubules. The first 80 amino acids of PEA-15 correspond to the death effector domain (DED), which is a domain found in proteins that regulate apoptotic signaling pathways. The DED domain is necessary for PEA-15 to block Ras suppression. Although PEA-15 is predominantly expressed in the central nervous system, low levels of PEA-15 are expressed in liver and kidney, and higher levels in muscle. PEA-15 is also referred to as PED, Phosphoprotein Enriched in Diabetes, for its elevated expression in type 2 diabetic patients. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PEA-15 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 51-130 mapping at the C-terminus of PEA-15 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 51-130 mapping at the C-terminus of PEA-15 of human origin |
| Entrez Gene | 8682 |
|---|
Application
Western blot analysis of PEA-15 expression in non-transfected: (A) and mouse PEA-15 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PEA-15 expression in U-87 MG whole cell lysate.
Application
Immunofluorescence staining of normal mouse kidney frozen section showing cytoplasmic staining.
Application
Western blot analysis of human recombinant PEA-15 fusion protein.| Application Notes | recommended for detection of PEA-15 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
