Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic and membrane staining of urothelial cells.Application
Western blot analysis of Ezrin expression in non-transfected: (A) and human Ezrin transfected: (B) 293T whole cell lysates.| Product Name | Ezrin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Ezrin of human origin |
| Modification Notes | Ezrin, Moesin and Radixin belong to a family of highly homologous actin-associated proteins that are localized just beneath the plasma membrane (1). The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins (2-5). Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor (5). Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78 (6). Moesin and Radixin share over 70% homology with Ezrin and are coexpressed within various cell types (7,8). Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Ezrin, Radixin, Moesin, and, to a lesser extent, NF2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Ezrin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Ezrin of human origin |
| Entrez Gene | 7430 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic and membrane staining of urothelial cells.
Application
Western blot analysis of Ezrin expression in non-transfected: (A) and human Ezrin transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Ezrin, Radixin, Moesin, and, to a lesser extent, NF2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
