Application
Western blot analysis of MTA2 expression in non-transfected 293T: (A), human MTA2 transfected 293T: (B) and ZR-75-1 (C) whole cell lysates.Application
Western blot analysis of MTA2 expression in ZR-75-1 (A) and HeLa (B) whole cell lysates and HeLa nuclear extract (C).Application
Western blot analysis of MTA2 expression in SK-BR-3 (A) and HeLa (B) nuclear extracts.| Product Name | MTA2 Antibody (H-170) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 499-668 mapping at the C-terminus of MTA2 of human origin |
| Modification Notes | MTA1 (metastasis-associated protein 1) is a component of the NURD (nucleosome remodeling and histone deacetylation) complex, which is associated with ATP-dependent chromatin-remodeling and histone deacetylase activity. MTA1 functions in conjunction with other components of NURD to mediate transcriptional repression as it facilitates the association of repressor molecules with the chromatin. Structurally, MTA1 contains a single SH3-binding motif and a zinc finger domain, along with a region similar to the co-repressor protein N-Cor. MTA1 is normally expressed at low levels in various tissues and is more highly expressed in testis. Overexpression of MTA1 correlates with tumor invasion and metastasis in various carcinomas including colorectal, gastrointestinal and breast carcinomas. Elevation of MTA1 levels in these tumors appears to enhance the metastases to lymph nodes, increase mammary cell motility and potentiate growth, and therefore may be an indicator for assessing the potential malignancies of various tumors. A similar protein, MTA2, also designated MTA1-L1 (MTA1-like protein 1), shares more than 55% sequence homology with MTA1 and is ubiquitously expressed. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MTA2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 499-668 mapping at the C-terminus of MTA2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 499-668 mapping at the C-terminus of MTA2 of human origin |
| Entrez Gene | 9219 |
|---|
Application
Western blot analysis of MTA2 expression in non-transfected 293T: (A), human MTA2 transfected 293T: (B) and ZR-75-1 (C) whole cell lysates.
Application
Western blot analysis of MTA2 expression in ZR-75-1 (A) and HeLa (B) whole cell lysates and HeLa nuclear extract (C).
Application
Western blot analysis of MTA2 expression in SK-BR-3 (A) and HeLa (B) nuclear extracts.| Application Notes | recommended for detection of MTA2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
