Application
Western blot analysis of E4BP4 expression in 293T (A) and HeLa (B) whole cell lysates.Application
Western blot analysis of E4BP4 expression in non-transfected 293T: (A), mouse E4BP4 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of E4BP4 expression in non-transfected: (A) and human E4BP4 transfected: (B) 293 whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of E4BP4 expression in rat liver (A) and mouse placenta (B) tissue extracts.| Product Name | E4BP4 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 163-462 mapping at the C-terminus of E4BP4 of human origin |
| Modification Notes | E4BP4, also known as NFIL3, functions as a transcriptional repressor and is a member of the basic leucine zipper (bZIP) transcription factor family. E4BP4 binds with high specificity to the E4 ATF, which is a DNA sequence traditionally targeted by the ATF/CREB family of transcription factors. A 65 amino acid segment located in the carboxy-terminus of E4BP4 interacts specifically with the TBP binding protein Dr1. In the suprachiasmatic nucleus, circadian center and liver, E4BP4 competes with PAR proteins for DNA binding via a reciprocating mechanism. The phase expression of E4BP4 correlates with the circadian cycle and represses transcription of genes otherwise activated by PAR transcription regulators. E4BP4 also plays an important role in an IL-3-mediated signaling pathway that is responsible for the survival of B cell progenitors. The gene encoding human E4BP4 maps to chromosome 9q22. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of E4BP4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 163-462 mapping at the C-terminus of E4BP4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 163-462 mapping at the C-terminus of E4BP4 of human origin |
| Entrez Gene | 4783 |
|---|
Application
Western blot analysis of E4BP4 expression in 293T (A) and HeLa (B) whole cell lysates.
Application
Western blot analysis of E4BP4 expression in non-transfected 293T: (A), mouse E4BP4 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of E4BP4 expression in non-transfected: (A) and human E4BP4 transfected: (B) 293 whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of E4BP4 expression in rat liver (A) and mouse placenta (B) tissue extracts.| Application Notes | recommended for detection of E4BP4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
