Application
Western blot analysis of Ral BP-1 expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of Ral BP-1 expression in non-transfected: (A) and mouse Ral BP-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Ral BP-1 expression in non-transfected: (A) and human Ral BP-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of human recombinant Ral BP-1.| Product Name | Ral BP-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 356-655 (deletion 581-592) mapping at the C-terminus of Ral BP-1 of human origin |
| Modification Notes | Ral A and Ral B constitute a distinct subfamily of Ras-related GTPases (i.e., GDP/GTP binding proteins). Ral proteins are activated by a unique nucleotide exchange factor, Ral GDS, and deactivated by a distinct GTPase-activating protein. Unlike Ras proteins, Ral A and Ral B fail to induce transformed foci when activated variants are expressed in various recipient cells. A potential downstream target of Ral, designated Ral BP-1, has been shown to contain a Rho-GTPase-activating domain. This Rho-GTPase-activating domain interacts preferentially with the Rho family member Cdc42. A Ras/Ral signaling pathway has been reported to mediate phospholipase D (PLD) activation by v-Src, thus indicating PLD as another downstream target of Ral A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ral BP-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 356-655 (deletion 581-592) mapping at the C-terminus of Ral BP-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 356-655 (deletion 581-592) mapping at the C-terminus of Ral BP-1 of human origin |
| Entrez Gene | 10928 |
|---|
Application
Western blot analysis of Ral BP-1 expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of Ral BP-1 expression in non-transfected: (A) and mouse Ral BP-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Ral BP-1 expression in non-transfected: (A) and human Ral BP-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of human recombinant Ral BP-1.| Application Notes | recommended for detection of Ral BP-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
