Application
Western blot analysis of PDE4D expression in rat skeletal muscle (A) and mouse skeletal (B) tissue extracts.Application
Western blot analysis of PDE4D expression in 293T whole cell lysate.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Product Name | PDE4D Antibody (H-69) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 741-809 mapping at the C-terminus of PDE4D of human origin |
| Modification Notes | Phosphodiesterases (PDE) hydrolyze cAMP to 5’AMP and thus play a critical role in the regulation of intracellular cAMP. Division of the PDE superfamily by sequence homology and enzymatic properties yields 11 PDE families (1). A unique upstream conserved region (UCR) characterizes the PDE4 family (2). Four separate genes (A-D) encode the PDE4 enzymes, and alternative splicing generates short or long isoforms of each gene. Long PDE4 isoforms contain both UCR1 and UCR2 while short PDE4 isoforms possess only UCR2. Both UCR domains are necessary for dimerization of PDE4 isoforms (3). The human PDE4D gene maps to chromosome 5q11.2 (4). The splice variants include isoforms PDE4D1-6 (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PDE4D isoforms 1-6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 741-809 mapping at the C-terminus of PDE4D of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 741-809 mapping at the C-terminus of PDE4D of human origin |
| Entrez Gene | 5144 |
|---|
Application
Western blot analysis of PDE4D expression in rat skeletal muscle (A) and mouse skeletal (B) tissue extracts.
Application
Western blot analysis of PDE4D expression in 293T whole cell lysate.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Application Notes | recommended for detection of PDE4D isoforms 1-6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
