Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic and nuclear staining of squamous epithelial cells.Application
Western blot analysis of endostatin fragment of COL18A1 expression in rat kidney (A), rat lung (B), rat liver (C), mouse pancreas (D) and rat pancreas (E) tissue extracts.Application
Immunofluorescence staining of methanol-fixed A549 cells showing cytoplasmic localization.| Product Name | COL18A1 Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1201-1340 of Collagen α1 Type XVIII of human origin |
| Modification Notes | Collagen Type XV (COL15 gene product) and XVIII (COL18 gene product) form the new subgroup MULTIPLEXIN, within the diverse family of collagens, which contains nineteen distinct types of collagens found in vertebrates. Both Collagen Type XV and Collagen XVIII are characterized by extensive interruptions in their collagenous sequences. Members of the MULTIPLEXIN subgroup contain polypeptides with multiple triple-helical domains separated and flanked by non-triple-helical regions. Type XV is predominantly expressed in internal organs such as adrenal gland, kidney and pancreas. Type XVIII encodes two different a1 chains, which have different signal peptides and variant N-terminal non-collagenous NC1 domains of 495 and 303 amino acids. The long variant NC1-434 Type XVIII mRNAs are promi-nently expressed in liver, while the variant NC1-303 mRNAs are predominantly expressed in heart, kidney, placenta, prostate, ovary, skeletal muscle and small intestine. Endostatin is a fragment of the C-terminal domain NC1 of Collagen Type XV and Type XVIII that inhibits angiogenesis and tumor growth. Unlike endostatin-XVIII, endostatin-XV does not bind zinc or heparin, however both endostatins can inhibit chorioallantoic membrane angiogenesis induced by basic FGF or VEGF. Collagen Type XV and XVIII are also widely present in basement membrane zones, suggesting their roles in basement membrane-stromal interactions and involvement with angiogenic and pathological processes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Collagen α1 Type XVIII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1201-1340 of Collagen &alpha 1 Type XVIII of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1201-1340 of Collagen α1 Type XVIII of human origin |
| Entrez Gene | 80781 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic and nuclear staining of squamous epithelial cells.
Application
Western blot analysis of endostatin fragment of COL18A1 expression in rat kidney (A), rat lung (B), rat liver (C), mouse pancreas (D) and rat pancreas (E) tissue extracts.
Application
Immunofluorescence staining of methanol-fixed A549 cells showing cytoplasmic localization.| Application Notes | recommended for detection of Collagen α1 Type XVIII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
